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轮状病毒的特定生长速率及细胞结合能力的测定
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
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轮状病毒的特定生长速率及细胞结合能力的测定

DOI:
10.3791/58821-v

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10:49 min

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January 28, 2019

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1Department of Civil and Environmental Engineering,Tohoku University, 2Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies,Tohoku University

Capitoli

  • 00:04Titolo
  • 00:42Cell Culture
  • 02:20Specific Growth Rate of Rotavirus
  • 06:12Cell-binding Assay
  • 08:53Results: Growth Curve of Rotavirus and Cell-binding Assay of Five Rhesus Rotavirus (RRV) Strains Purified from Plaques
  • 09:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了两个方案, 一个用于测量特定的生长速率, 另一个是利用斑块分析和 rt-qpcr 来测量轮状病毒的细胞结合能力。这些协议可用于确认轮状病毒株之间表型的差异。

Tags

RotavirusPlaque AssayCell-binding AssayMA104 Cell LineCell CultureVirus TiterDisinfectant-resistant StrainsPhenotypic ChangeVirus PropagationVirus ActivationMultiplicity Of Infection (MOI)

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