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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
 

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

Article DOI: 10.3791/58871-v • 09:50 min • February 11th, 2019
February 11th, 2019 •
1,3, 2, 1,3, 1,3, 1,3
1Department of Internal Medicine 1, University Erlangen-Nürnberg, University Hospital Erlangen, 2Institute of Pathology, Department of Nephropathology, University Hospital Erlangen, 3Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen

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Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.

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