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小鼠颅面组织和未切除骨骼的组织制备及免疫染色

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Please note that all translations are automatically generated. Click here for the English version.

小鼠颅面组织和未切除骨骼的组织制备及免疫染色

DOI:

10.3791/59113-v

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10:03 min

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May 10, 2019

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Jingwen Yang*^1,2, Haichun Pan*², Yuji Mishina

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology,Wuhan University, ²Department of Biologic and Materials Sciences, School of Dentistry,University of Michigan

Capitoli

00:04Titolo
01:13Preparation of Embryonic Tissues
03:38Preparation of Postnatal Undecalcified Hard Tissues
04:15Cryosectioning
05:18Immunofluorescence Staining
06:50Results: Immunostained Mouse Craniofacial Tissues and Undecalcified Bone
09:03Conclusion

Summary

Traduzione automatica

在这里,我们提出了一个详细的协议,以小鼠颅面组织为例,通过免疫染色来检测和量化颅面形态形成/发病机制期间的蛋白质水平。此外,我们描述了一种制备和冷冻方法,从幼鼠的未分化硬组织免疫染色。

Tags

Immunofluorescence Immunostaining Undecalcified Bone Antigen Retrieval Decalcification Cryoprotection Embedding Freezing Sectioning PBS PFA EDTA Sucrose OCT Compound

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