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高盐抗原表达树突状细胞的分离和采用转移
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
DOI:

09:29 min

March 05, 2019

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Capitoli

  • 00:04Titolo
  • 00:52Isolation of Spleens from Mice
  • 01:27Generation of Single Cell Suspensions from Spleens
  • 02:39Isolation of CD11c+ DCs from Splenic Single Cell Suspension
  • 03:41Magnetic Separation of CD11c+ DCs
  • 04:51Adoptive Transfer of High Salt Treated CD11c+ DCs
  • 06:56Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
  • 08:52Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 以分离树突状细胞从小鼠的脾脏通过磁性细胞分类和随后采用转移到天真的小鼠。选择高盐活化树突状细胞模型, 解释采用转移和流式细胞术的分步过程。

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