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Biochemistry

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JoVE Journal Biochemistry
Nitropeptide profilering en identificatie geïllustreerd door angiotensine II
 
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Nitropeptide profilering en identificatie geïllustreerd door angiotensine II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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Proteomic profilering van tyrosine-nitraat eiwitten is een uitdagende techniek vanwege de geringe overvloed van de 3-nitrotyrosine modificatie. Hier beschrijven we een nieuwe aanpak voor nitropeptide verrijking en profilering met behulp van angiotensine II als model. Deze methode kan worden uitgebreid voor andere in vitro -of in vivo -systemen.

Tags

Biochemie Nitropeptide angiotensine II nitrotyrosine dimethyl etikettering natrium dithionate Biotine etikettering verrijking
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