Rivista
/
/
在自抗性混合奇美体模型中, 光激活询问个体自反应萌发中心
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
DOI:

11:12 min

April 11, 2019

, ,

Capitoli

  • 00:04Titolo
  • 01:16Bone Harvest
  • 02:26Bone Marrow Cell Extraction
  • 03:16Bone Marrow Recipient Reconstitution
  • 04:48Popliteal Lymph Node Labelling and Harvest
  • 06:54Single Splenic Germinal Center Identification
  • 08:43Results: Representative Germinal Center Photoactivation
  • 10:19Conclusion

Summary

Traduzione automatica

该方案描述了混合小鼠骨髓嵌合体与自发自身免疫性萌发中心的生成, 其中自反应淋巴细胞携带光可活性的绿色荧光蛋白 (PA-GFP) 记者。这提供了将细胞在组织中的位置与下游分子和功能分析联系起来的能力。

Video correlati

Read Article