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Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
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JoVE Journal Immunologia e infezioni
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

DOI:
10.3791/59400-v

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08:47 min

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April 05, 2019

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, ,
1Department of Biochemistry and Molecular Biology, Center for Molecular Medicine and Complex Carbohydrate Research Center,University of Georgia

Capitoli

  • 00:04Titolo
  • 01:21Culture, Differentiation, and Validation of HL-60 Cells
  • 02:30Preparation of Bacterial Stock Samples
  • 03:53Bacterial Treatment and Culture
  • 04:43HL-60 Bacterial Co-culture
  • 05:46Sample Plating and Overnight Incubation
  • 06:55Results: Determining Bacterial Cell Viability using OPKA
  • 07:55Conclusion

Summary

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This opsonophagocytic killing assay is used to compare the ability of phagocytic immune cells to respond to and kill bacteria based on different treatments and/or conditions. Classically, this assay serves as the gold standard for assessing effector functions of antibodies raised against a bacterium as opsonin.

Tags

Opsonophagocytic Killing AssayImmunotherapeuticAntibacterial ActivityPhagocytosisStreptococcus PneumoniaHL60 CellsBacterial StocksFlow CytometryRPMIFetal Bovine SerumDimethyl SulfoxideDMFBacterial StrainGlycerol

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