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JoVE Journal Neuroscience
High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
 

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Article DOI: 10.3791/59402-v • 09:42 min • April 30th, 2019
April 30th, 2019 •
1, 1, 1, 1, 1
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health

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Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.

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Keywords: 3D Imaging Rabies Virus Brain Tissue Immunostaining Solvent Clearing Virus Infection Cellular Context Tissue Fixation Vibratome Sectioning Methanol Pretreatment Bleaching Immunostaining Permeabilization Blocking Primary Antibody Labeling
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