Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Analysis of Cardiac Chamber Development During…
JoVE Journal
Developmental Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
 

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

Capitoli

Riepilogo
Automatic Translation

We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

Tags

Keywords: Cardiac Chamber Development Mouse Embryogenesis Whole Mount Epifluorescence MLC-2v-tdTomato Heart Tube Formation Looping Chamber Formation Fluorescent Reporter Dissection Genotyping Epifluorescent Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter