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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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Neuroscienze
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JoVE Journal Neuroscienze
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

DOI:
10.3791/59433-v

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05:51 min

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May 23, 2019

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, , ,
1Department of Neurosciences,University of California, San Diego

Capitoli

  • 00:04Titolo
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

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Traduzione automatica

This protocol details a procedure in which human neuronal cultures are transduced with lentiviral constructs coding for mutant human tau. Transduced cultures display tau aggregates and associated pathologies.

Tags

Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

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