An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Christian S. Thudium; Amalie Engstrom; Solveig  S. Groen; Morten  A. Karsdal; Anne-Christine Bay-Jensen

doi:10.3791/59467

Rivista

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Biologia

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An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

JoVE Journal
Biologia

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JoVE Journal Biologia

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

DOI:

10.3791/59467-v

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07:10 min

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November 03, 2019

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Christian S. Thudium*¹, Amalie Engstrom*^1,2, Solveig S. Groen, Morten A. Karsdal, Anne-Christine Bay-Jensen

¹Nordic Bioscience, ²Department of Biomedical Sciences,University of Copenhagen

Capitoli

00:04Titolo
00:50Tissue Isolation
02:35Bovine Cartilage Explant Treatment and Assessment of Metabolic Activity
04:21Results: Analysis of Cartilage Remodeling in Bovine Knee Explants
06:14Conclusion

Summary

Traduzione automatica

Here, we present a protocol describing isolation and culturing of cartilage explants from bovine knees. This method provides an easy and accessible tool to describe tissue changes in response to biological stimuli or novel therapeutics targeting the joint.