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Developmental Biology

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JoVE Journal Developmental Biology
Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode
 

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

Article DOI: 10.3791/59487-v • 07:10 min • March 27th, 2019
March 27th, 2019 •
1, 1, 2, 3
1School of Biosciences, University of Melbourne, 2Melbourne School of Psychological Sciences, University of Melbourne, 3Department of Optometry and Vision Sciences, University of Melbourne

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Here, we present a protocol that simplifies the measurement of light evoked electroretinogram responses from larval zebrafish. A novel cone-shaped sponge-tip electrode can help to make the study of visual development in larval zebrafish using the electroretinogram ERG easier to achieve with reliable outcomes and lower cost.

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Keywords: Electroretinogram ERG Larval Zebrafish Cone-shaped Sponge-tip Electrode Visual Development Genetic Disease Models Electrode Manufacture Dark Adaptation Ringer's Buffer Photovoltaic Artifacts
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