Journal
/
/
Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
DOI:

05:58 min

July 17, 2019

, , , ,

Chapters

  • 00:04Title
  • 00:56Saccharomyces cerevisiae Preparation
  • 02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
  • 02:51Data Analysis
  • 03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
  • 05:26Conclusion

Summary

Automatic Translation

Dit artikel beschrijft een FRET-based flow cytometrie protocol voor het kwantificeren van eiwit zelf montage in zowel S. cerevisiae en HEK293T cellen.

Related Videos

Read Article