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Chapters
- 00:05Title
- 02:03Testis Dissociation and Hoechst 33342 Staining of Testicular Cells
- 06:03FACS Setup and Purification
- 08:30Results of Flow Cytometry Purification
- 09:58Conclusion
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
