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Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
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JoVE Journal Medicina
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

DOI:
10.3791/59608-v

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09:34 min

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September 26, 2019

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, , ,
1Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS),Saarland University, 2Institute for Clinical and Experimental Surgery,Saarland University

Capitoli

  • 00:04Titolo
  • 01:05Primary Cell Culture and Small Interfering RNA (siRNA) Transfection
  • 02:56In Vitro Wound Healing (Scratch Migration) Assay
  • 04:13In Vivo Skin Wound Healing Assay
  • 07:11Results: Representative In Vitro and In Vivo Wound Healing
  • 08:43Conclusion

Summary

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Here, we present a protocol for an in vitro scratch assay using primary fibroblasts and for an in vivo skin wound healing assay in mice. Both assays are straightforward methods to assess in vitro and in vivo wound healing.

Tags

Scratch Migration AssayDorsal Skinfold ChamberWound HealingIn VitroIn VivoCell MigrationSkin WoundPharmacologically Active CompoundsFibroblastsGenotypeLipid-based Transfection ReagentSiRNACell CultureConfluencyScratch Wound

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