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JoVE Journal
Immunology and Infection
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Studying Organelle Dynamics in B Cells During Immune Synapse Formation
 

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Article DOI: 10.3791/59621-v 15:39 min June 1st, 2019
June 1st, 2019

Capitoli

Riepilogo

Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.

Tags

Keywords: Organelle Dynamics B Cells Immune Synapse Formation Immunofluorescence Antigen-coated Beads BCR Ligand Cell Activation Endolysosome MHC Class II T Helper Cells
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