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Biochemistry

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JoVE Journal Biochemistry
Anvendelse af Biolayer interferometri (BLI) til undersøgelse af protein-protein interaktioner i transkriptionen
 
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Anvendelse af Biolayer interferometri (BLI) til undersøgelse af protein-protein interaktioner i transkriptionen

Article DOI: 10.3791/59687-v • 07:18 min • July 26th, 2019
July 26th, 2019 •
1,2, 3, 1,2
1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, 3Department of Plant Biology, School of Environmental and Biological, Rutgers University

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Interaktioner af transkriptionsfaktorer (TFS) med RNA-polymerase er normalt undersøgt ved hjælp af rulle assays. Vi anvender en biolayer interferometri (bli) teknologi til at karakterisere samspillet mellem GRGA med Chlamydia RNA polymerase. Sammenlignet med rulle assays, registrerer bli real-time Association og dissociation, giver højere følsomhed, og er meget kvantitativ.

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Denne måned i JoVE Biolayer interferometri klamydia CT504 TC0791 GrgA protein-protein interaktion transkriptionsfaktorer transkriptionsregulering
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