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Developmental Biology

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JoVE Journal Developmental Biology
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
 

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Article DOI: 10.3791/59743-v • 08:36 min • July 16th, 2019
July 16th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park

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Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.

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Keywords: TILLING Mutagenesis EMS Small Grain Crops Functional Genomics Gene Discovery Cel-1 Assay Mutation Detection Dosage Optimization M2 Plants
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