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Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

DOI:
10.3791/59749-v

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09:17 min

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July 12, 2019

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, , , , , , , , , , ,
1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 3Department of Neurosurgery,University of Mississippi Medical Center

Capitoli

  • 00:04Titolo
  • 00:47Adult Mouse Hind Limb Distal P3 Amputation
  • 02:01Digit Collection and Tissue Preparation
  • 02:47Immunostaining for Blastema Formation and Intramembranous Ossification
  • 04:30Sequential In Vivo Microcomputed Tomography (microCT) Imaging
  • 05:50Reconstructed Bone Image Analysis
  • 08:15Results: Representative Regenerative Bone Analyses
  • 08:47Conclusion

Summary

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Here, we present a protocol of adult mouse terminal phalanx amputation to investigate mammalian blastema formation and intramembranous ossification, analyzed by fluorescent immunohistochemistry and sequential in-vivo microcomputed tomography.

Tags

Mouse Digit AmputationBlastema FormationIntramembranous OssificationEpimorphic RegenerationPreclinical ModelRevascularizationCD-1 MouseMedial Digit SurfaceTerminal PhalanxMiddle PhalanxBuffered Zinc Formalin FixativeMicrotomeHistological Adhesive Solution

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