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Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
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1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 3Department of Neurosurgery,University of Mississippi Medical Center
Capitoli
- 00:04Titolo
- 00:47Adult Mouse Hind Limb Distal P3 Amputation
- 02:01Digit Collection and Tissue Preparation
- 02:47Immunostaining for Blastema Formation and Intramembranous Ossification
- 04:30Sequential In Vivo Microcomputed Tomography (microCT) Imaging
- 05:50Reconstructed Bone Image Analysis
- 08:15Results: Representative Regenerative Bone Analyses
- 08:47Conclusion
Hier stellen wir ein Protokoll der erwachsenen Maus-Terminal-Phalanx-Amputation vor, um die Explosion von Säugetieren und die intramembranöse Ossifikation zu untersuchen, die durch fluoreszierende Immunhistochemie und sequenzielle In-vivo-Mikrocomputertomographie analysiert wird.
