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Amputation et régénération des chiffres de souris adultes : un modèle simple pour étudier la formation d'blastema de mammifères et l'ossification intramembrane
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1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 3Department of Neurosurgery,University of Mississippi Medical Center
Capitoli
- 00:04Titolo
- 00:47Adult Mouse Hind Limb Distal P3 Amputation
- 02:01Digit Collection and Tissue Preparation
- 02:47Immunostaining for Blastema Formation and Intramembranous Ossification
- 04:30Sequential In Vivo Microcomputed Tomography (microCT) Imaging
- 05:50Reconstructed Bone Image Analysis
- 08:15Results: Representative Regenerative Bone Analyses
- 08:47Conclusion
Ici, nous présentons un protocole de l'amputation de phalange terminale de souris adulte pour étudier la formation de blastma de mammifères et l'ossification intramembrane, analysée par l'immunohistochemistry fluorescent et la tomographie microcomputed in-vivo séquentielle.
