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通过有针对性的测序方法进行比较病变分析

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JoVE Journal Comportamento

Comparative Lesions Analysis Through a Targeted Sequencing Approach

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通过有针对性的测序方法进行比较病变分析

DOI:

10.3791/59844-v

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08:16 min

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November 05, 2019

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Caterina Vicentini, Andrea Mafficini, Michele Simbolo, Matteo Fassan, Pietro Delfino, Rita Teresa Lawlor, Borislav Rusev, Aldo Scarpa², Vincenzo Corbo²

¹ARC-Net Research Centre,University and Hospital Trust of Verona, ²Department of Diagnostics and Public Health, Section of Pathology,University and Hospital Trust of Verona, ³Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit,University of Padua

Capitoli

00:04Titolo
00:48Library Pooling and Sequencing Run
02:28Sample Library Dilution and Loading
03:00Next Generation Sequencing
03:43Mutation and Copy-Number Variation (CNV) Analysis
05:00Somatic Mutation Indexing
05:36Results: Representative Comparative Lesion Analyses
07:16Conclusion

Summary

Traduzione automatica

本文介绍了一种识别特定患者不同标本之间的克隆和亚克隆变化的方法。虽然此处描述的实验侧重于特定的肿瘤类型，但该方法广泛适用于其他实体肿瘤。

Tags

Targeted Sequencing Genetic Relatedness Molecular Alterations Disease Progression Intratumor Heterogeneity Anti-tumor Strategies Solid Tumor Types Sequencing Run Ion Proton System Ion Gene Studio S5 System Ion AmpliSeq 2.0 Library Kit Ion Chef Coverage Analysis Plugin GRCH37HG19 Genome Barcode Set Clonal Amplification Next Generation Sequencing

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