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Journal / Genetics / 通过CAS9蛋白-gRNA复合物电穿孔直接基因敲除Axolotl脊髓神经干细胞…
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JoVE Journal Genetics
通过CAS9蛋白-gRNA复合物电穿孔直接基因敲除Axolotl脊髓神经干细胞
 
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通过CAS9蛋白-gRNA复合物电穿孔直接基因敲除Axolotl脊髓神经干细胞

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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这里介绍的一个协议,通过将CAS9-gRNA复合物注射到脊髓中央管中,然后进行电穿孔,在axolotl脊髓中执行时间和空间限制基因敲除。

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遗传学 问题 149 轴球 脊髓 再生 CRISPR-Cas9 神经干细胞 基因敲除
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