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成人脑切片长期抑郁症诱导评估
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JoVE Journal Neuroscienze
Assessment of Long-term Depression Induction in Adult Cerebellar Slices
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成人脑切片长期抑郁症诱导评估

DOI:
10.3791/59859-v

•

09:30 min

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October 16, 2019

•

,
1Laboratory for Behavioral Genetics, Center for Brain Science,RIKEN, 2Senior Adviser’s Office, Center for Brain Science,RIKEN

Capitoli

  • 00:04Titolo
  • 00:50Brain Dissection and Trimming
  • 02:52Brain Slicing
  • 04:10Whole-Cell Patch-Clamp Recording
  • 06:10Long Term Depression (LTD) Induction
  • 07:12Results: Representative Purkinje Cell Voltage and Current Traces During Conjunctive Stimulation
  • 08:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

在一些基因操纵的动物中，使用单一协议可能无法诱导小脑Purkinje细胞中的LTD，并且LTD和运动学习之间可能存在差异。在基因操纵的动物中，需要多种协议来评估LTD诱导。显示了标准协议。

Tags

LTD InductionCerebellar SlicesGene ManipulationMotor LearningACSFTetrodotoxinBrain DissectionPurkinje CellsCerebellum SlicingCutting ACSFOxygen-carbon Dioxide Gas

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