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Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues

DOI:
10.3791/60138-v

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08:42 min

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October 26, 2021

•

,
1Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention

Capitoli

  • 00:04Introduction
  • 01:20Formalin Fixation of Tissues
  • 01:51Tissue Processing
  • 02:24Preparation of Materials & Staining Dishes
  • 03:14Deparaffinization and Tissue Rehydration
  • 04:05Proteolytic Antigen Retrieval
  • 04:30Staining Procedure
  • 06:22Results: Analysis of the Immunohistochemistry Test
  • 07:57Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present an immunohistochemistry test protocol for the detection of rabies virus antigen as an alternative diagnostic test for formalin-fixed tissues.

Tags

ImmunohistochemistryLyssavirusAntigen DetectionRabies VirusPost-exposure ProphylaxisParaffin EmbeddingMicrotomeAEC SubstrateProteaseRinse Buffer

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