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恒流中原发性人类内皮细胞的肺炎球菌感染
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
DOI:

09:34 min

October 31, 2019

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Capitoli

  • 00:04Titolo
  • 01:29Endothelial Cell Cultivation of HUVEC, Induction of VWF (Von Willebrand Factor)-release and Visualization of Multimerized VWF Strings
  • 05:53Microscopic Evaluation of Bacterial Attachment to VWF-strings in Flow in Real Time and after Sample Fixation
  • 07:12Results: Analyses of Bacterial Attachment to VWF Strings
  • 08:33Conclusion

Summary

Traduzione automatica

本研究描述了在定义的流动条件下,在剪切应力下在分化人类原发内皮细胞表面产生的对冯·威勒布兰德因子字符串的肺炎球菌依从的微观监测。通过应用微分免疫染色程序,此协议可扩展到特定细胞结构的详细可视化和细菌定量。

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