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Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

DOI:
10.3791/60424-v

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07:41 min

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October 23, 2019

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, , ,
1Department of Physiology and Pharmacology,Karolinska Institutet, 2Institute for Regenerative Medicine,Sechenov First Moscow State Medical University (Sechenov University), 3Department of Women’s and Children’s Health, Karolinska Institutet and Pediatric Endocrinology Unit,Karolinska University Hospital

Capitoli

  • 00:04Titolo
  • 01:07Tissue Fixation and Processing
  • 02:50Sectioning and Slide Preparation
  • 04:09Confocal Microscopy
  • 05:42Results: Confetti Images from a Col2CreERT: Confetti Mouse
  • 06:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

This method describes the use of the R26R-Confetti (Confetti) mouse model to study mineralized tissues, covering all steps from the breeding strategy to the image acquirements. Included is a general protocol that can be applied to all soft tissues and a modified protocol that can be applied to mineralized tissues.

Tags

Clonal Genetic TracingConfetti MouseFluorescent ProteinsRegenerative MedicineBiologia dello sviluppoFormaldehydeEDTASucroseCryosectioningOCT Compound

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