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Bioengineering

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JoVE Journal Bioengineering
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
 

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Article DOI: 10.3791/60577-v • 09:28 min • May 18th, 2020
May 18th, 2020 •
1, 1, 1, 1
1Institute for Technical Chemistry, Gottfried-Wilhelm-Leibniz Universität Hannover

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Here, we present a detailed procedure to run a Design of Experiment in an automated micro-bioreactor followed by cell harvest and protein quantification using a Protein A column.

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Process Optimization High Throughput Automated Micro-bioreactors Chinese Hamster Ovary Cell Cultivation Micro-bioreactor Software Design Of Experiments Analyzers Auxiliary Valves Modet Data Analysis Experiments Small Scale Pre-culture Procedure Amber Self-culture Software New Experiment Culture Station Vessels Auto-add DOE Tags Addition Of Media Anti-foam Inoculum Feed Glucose
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