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Journal / Bioengineering / Generic Protocol for Optimization of Heterologous …
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Bioengineering

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JoVE Journal Bioengineering
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
 

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Article DOI: 10.3791/56234-v • 06:24 min • December 15th, 2017
December 15th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2,4
1IBG-1: Biotechnology, Forschungszentrum Jülich, 2Research Center Jülich, Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 4Institute for Biotechnology, RWTH Aachen University
* These authors contributed equally

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This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.

Tags

Optimization Heterologous Protein Production Automated Microbioreactor Technology Experimental Design Cultivation Media Bioprocess Development Strain Phenotyping Metabolite Production Modeling Development Times Study Conception Methods Definition Sterilization Deep Well Plates Seed Culture BHI Medium Kanamycin Robotic Work Table
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