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JoVE Journal Bioengineering
Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
 

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches.

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Here Are The Key Keywords Extracted From The Given Text: 3D Bioprinting Adipose-derived Stem Cells Spheroids Automated Production Microbiology Contamination Labor Hours Software Parameters Cell Culture Media 3D Cell Culture Stem Cell Suspension ASC Pellet DMEM-Low FBS Micromolded Agarose Ultra Pure Agarose Silicone Mold 12-well Plastic Plate Laminar Flow Carbon Dioxide LabWare Editor Virtual Work Table Pipettes Tip Boxes Plastic Tubes Plates
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