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Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
 

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Article DOI: 10.3791/60652-v • 05:55 min • December 21st, 2019
December 21st, 2019 •
1,2, 1, 1
1Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 2Research Fellow of Japan Society for the Promotion of Science

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Here, we present a method for screening anti-hepatitis B viral agents that inhibit the HBx-DDB1 interaction using a split luciferase assay system. This system allows easy detection of protein-protein interactions and is suitable for identifying inhibitors of such interactions.

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Keywords: HBX-DDB1 Interaction Split Luciferase Assay Viral Transcription CccDNA Protein-protein Interactions Drug Targets Antiviral Therapy HPV HEK293T Cells Transfection
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