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Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
 

Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

Article DOI: 10.3791/60679-v • 10:14 min • February 16th, 2020
February 16th, 2020 •
*1, *1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 1, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences, Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University
* These authors contributed equally

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Automatic Translation

In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.

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Keywords: Inguinal Subcutaneous White Adipose Tissue ISWAT Islet Transplantation Type I Diabetes Murine Islets Pancreas Digestion Islet Isolation Collagenase DNase I Islet Engraftment
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