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Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
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JoVE Journal Ricerca sul cancro
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

DOI:
10.3791/60687-v

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10:32 min

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December 19, 2019

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, ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Capitoli

  • 00:04Titolo
  • 00:32Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
  • 01:59Tail Vein Injection of Labeled Cancer Cells
  • 04:17Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
  • 06:18Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
  • 08:01Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
  • 09:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

The described approach combines experimental tail vein metastasis assays with in vivo live animal imaging to allow real-time monitoring of breast cancer metastasis formation and growth in addition to the quantification of metastasis number and size in the lungs.

Tags

Keywords: Tail Vein Metastasis AssayReal-time In Vivo ImagingBreast CancerMetastatic ColonizationMetastasis BurdenLungT1 CellsLuciferaseFluorescent ProteinViral TransductionCell CultureCell CountingCell InjectionAnimal Model

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