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尾静脈転移アッセイとリアルタイムインビボイメージングの併用による乳癌転移性コロニー形成と肺の負担の定量化
JoVE Journal
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JoVE Journal Ricerca sul cancro
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
DOI:

10:32 min

December 19, 2019

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Capitoli

  • 00:04Titolo
  • 00:32Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
  • 01:59Tail Vein Injection of Labeled Cancer Cells
  • 04:17Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
  • 06:18Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
  • 08:01Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
  • 09:36Conclusion

Summary

Traduzione automatica

記述されたアプローチは、実験的な尾静脈転移アッセイと生体内生動物イメージングを組み合わせることで、肺の転移数と大きさの定量に加えて、乳癌転移の形成と増殖をリアルタイムでモニタリングすることを可能にする。

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