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基于 TurboID 的接近标签,用于蛋白质-蛋白质相互作用网络的植物中识别
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
DOI:

07:02 min

May 17, 2020

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Capitoli

  • 00:04Introduction
  • 01:01Agroinfiltration and Leaf Sample Collection
  • 01:59Extraction of Leaf Total Protein and Removal of Free Biotin
  • 04:11Enrichment of Biotinylated Proteins
  • 05:53Results: Western Blot of Protein Extracts
  • 06:25Conclusion

Summary

Traduzione automatica

这里描述的是一种接近标记方法,用于识别尼科蒂亚娜本查米亚叶组织中NLR免疫受体的TIR域的相互作用伙伴。还提供了一个详细的协议,用于识别其他感兴趣的蛋白质之间的相互作用使用这种技术在尼科蒂亚纳和其他植物物种。

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