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Genetics

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JoVE Journal Genetics
Transkriptom-Wide Profilering av protein-RNA interaktioner genom cross-linking och immunoprecipitation medierad av FLAG-Biotin Tandem Rening
 
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Transkriptom-Wide Profilering av protein-RNA interaktioner genom cross-linking och immunoprecipitation medierad av FLAG-Biotin Tandem Rening

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Här presenterar vi en modifierad CLIP-seq protokoll som kallas FBIOCLIP-seq med FLAG-biotin tandem rening för att fastställa RNA-mål för RNA-bindande proteiner (RBPs) i däggdjursceller.

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Genetik FBIOCLIP-seq RNA-biologi RNA-bindande protein protein-RNA interaktion WDR43 LIN28
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