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人体器官性心脏切片双电压和钙光学映射的临床前心脏电生理评估

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Bioingegneria

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JoVE Journal Bioingegneria

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

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人体器官性心脏切片双电压和钙光学映射的临床前心脏电生理评估

DOI:

10.3791/60781-v

•

09:35 min

•

June 16, 2020

•

Sharon A. George*¹, Jaclyn A. Brennan*¹, Igor R. Efimov

¹Department of Biomedical Engineering,The George Washington University

Capitoli

00:04Introduction
00:29Optical Mapping System Setup
01:16Slicing Protocol
02:41Static Slice Culture
03:22Optical Mapping
05:01Data Processing
07:24Results: Representative Transmembrane Potential and Calcium Transient Mapping
08:38Conclusion

Summary

Traduzione automatica

该协议描述了用于临床前药物测试的人体心脏切片切片和培养过程，并详细介绍了光学映射用于同时记录这些切片的跨膜电压和细胞内钙信号的过程。

Tags

Keywords: Optical Mapping Cardiac Electrophysiology Human Organotypic Cardiac Slices Voltage Dye Calcium Dye Action Potentials Intracellular Calcium Transients Perfusion System Vibratome Cardioplegia Tyrode’s Recovery Solution Preclinical Physiological Drug Testing

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