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在心肌梗死小鼠模型中传递修正的mRNA
JoVE Journal
Genetica
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JoVE Journal Genetica
Delivery of Modified mRNA in a Myocardial Infarction Mouse Model
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在心肌梗死小鼠模型中传递修正的mRNA

DOI:
10.3791/60832-v

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06:03 min

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June 11, 2020

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, , , , , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Capitoli

  • 00:04Introduction
  • 01:06Preparation of modRNA Injection
  • 01:32Myocardial Infarction Surgery
  • 04:07Results: Validation of modRNA Delivery
  • 05:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议提供了一种简单和连贯的方法，在小鼠心肌梗死后，使用modRNA暂时调节感兴趣的基因。

Tags

Modified RNAGene TransferMyocardial InfarctionIschemia ReperfusionTransverse Aortic ConstrictionIntratracheal IntubationLAD LigationIntramuscular Injection

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