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切り替え可能なプロトポルフィリンIX依存性活性酸素種のin situ光生成によるマウス皮膚における幹細胞ニッチの刺激と組織再生
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JoVE Journal
Biology
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
Please note that all translations are automatically generated.
Click here for the English version.
切り替え可能なプロトポルフィリンIX依存性活性酸素種のin situ光生成によるマウス皮膚における幹細胞ニッチの刺激と組織再生
DOI:
10.3791/60859-v
•
10:05 min
•
May 08, 2020
•
Jesús Espada
2
,
Elisa Carrasco
,
María I. Calvo-Sánchez
4
,
Sandra Fernández-Martos
,
Juan José Montoya
1
Ramón y Cajal Institute for Health Research (IRYCIS)
,
Ramón y Cajal University Hospital
,
2
Centro Integrativo de Biología y Química Aplicada (CIBQA)
,
Universidad Bernardo O’Higgins
,
3
Department of Molecular Biology, Faculty of Sciences
,
Universidad Autónoma de Madrid (UAM)
,
4
Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales
,
Universidad Francisco de Vitoria
,
5
Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine
,
Complutense University
Chapters
00:04
Introduction
01:26
Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
02:58
Transient Nonlethal Reactive Oxygen Species (ROS) Production
04:37
BrdU Detection in Mouse Tail Skin
05:38
ROS Detection in Mouse Tail Skin
07:16
Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
09:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目的は、マウスの皮膚で非致死レベルの活性酸素種(ROS)の一時的なin vivo産生を誘導し、組織内の生理学的応答をさらに促進することです。
Tags
Stem Cell Niche
Reactive Oxygen Species
ROS
Protoporphyrin IX
Photodynamic Therapy
Skin
Hair Growth
Wound Healing
BrdU
Labeling
In Vivo
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