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切り替え可能なプロトポルフィリンIX依存性活性酸素種のin situ光生成によるマウス皮膚における幹細胞ニッチの刺激と組織再生
JoVE Journal
Biology
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JoVE Journal Biology
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
DOI:

10:05 min

May 08, 2020

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Chapters

  • 00:04Introduction
  • 01:26Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
  • 02:58Transient Nonlethal Reactive Oxygen Species (ROS) Production
  • 04:37BrdU Detection in Mouse Tail Skin
  • 05:38ROS Detection in Mouse Tail Skin
  • 07:16Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
  • 09:00Conclusion

Summary

Automatic Translation

このプロトコルの目的は、マウスの皮膚で非致死レベルの活性酸素種(ROS)の一時的なin vivo産生を誘導し、組織内の生理学的応答をさらに促進することです。

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