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Developmental Biology

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JoVE Journal Developmental Biology
Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
 

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Article DOI: 10.3791/60888-v • 08:59 min • April 3rd, 2020
April 3rd, 2020 •
*1,2,3, *1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3,4,5
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim
* These authors contributed equally

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This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.

Tags

Lithography-free Single-cell Micropatterning Laser-cut Stencils Stencil-based Method Reusable Stencils Inexpensive Process Customizable Process Non-engineer Researchers Cell Shape Area-induced Mechanotransduction Physiological Contexts Pathological Contexts High-throughput Drug Screening Disease Modeling Detachment Of Coverslip Drying Hydrogel Polyacrylamide Hydrogel Solution Photoinitiator Solution Basement Membrane Matrix Protein Stock Solution
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