Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Characterization of Adult Cardiac…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
 

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Article DOI: 10.3791/60909-v • 10:45 min • March 12th, 2020
March 12th, 2020 •
1, 1, 1,2, 1
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

Capitoli

Riepilogo
Automatic Translation

Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

Tags

Keywords: Cardiac Fibroblasts Myofibroblasts Isolation Characterization Flow Cytometry Bead-based Isolation Tissue Dissection Collagenase Digestion Cell Culture
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter