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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af tonsillar mononukleare celler til at studere Ex Vivo medfødte immunrespons i en human slimhinde lymfoide væv
 
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Isolering af tonsillar mononukleare celler til at studere Ex Vivo medfødte immunrespons i en human slimhinde lymfoide væv

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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I denne protokol forklarer vi, hvordan man nemt behandler og kultur tonsillar mononukleare celler fra raske mennesker, der gennemgår delvis kirurgisk tonsillektomi for at studere medfødte immunrespons ved aktivering, efterligne virusinfektion i slimhindevæv.

Tags

Immunologi og infektion slimhinde-associeret lymfoide væv tonsil tonsillar mononuklear celle immunitet ex vivo cellekultur cytokin vært-patogen interaktion
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