Rivista
/
Neuroscienze
/
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

DOI:
10.3791/60989-v

•

09:40 min

•

April 02, 2020

•

, , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capitoli

  • 00:04Introduction
  • 00:38Preparation of Dil/Tungsten Bead Tubing
  • 02:55Preparation of Brain Sections
  • 04:20Ballistic Labeling and Visualization of Brain Sections
  • 06:16Cell Culture Method
  • 07:12Results: Analysis of Pyramidal Neurons Using Ballistic Labeling Technology
  • 08:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a protocol to label and analyze pyramidal neurons, which is critical for evaluating potential morphological alterations in neurons and dendritic spines that may underlie neurochemical and behavioral abnormalities.

Tags

Ballistic LabelingPyramidal NeuronsBrain SlicesPrimary Cell CulturePVP SolutionTungsten Microcarrier BeadsLipophilic DiIC18(3) DyeMethylene ChlorideSonicationNitrogen Gas DryingRat Brain PerfusionPBSPFA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60989?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/it/60989?article_type=v

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
check_url/it/60989?article_type=v

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
check_url/it/60989?article_type=v

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
check_url/it/60989?article_type=v

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
check_url/it/60989?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/it/60989?article_type=v

Primary Culture of Mouse Dopaminergic Neurons
check_url/it/60989?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/it/60989?article_type=v

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
check_url/it/60989?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices

Read Article