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Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
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JoVE Journal Bioingegneria
Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

DOI:
10.3791/60990-v

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14:28 min

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July 15, 2020

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, , , , , ,
1Université de Lorraine, CNRS, IMoPA F54000 Nancy France, 2Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

Capitoli

  • 00:00Introduction
  • 01:06Download Icy and the “Substructure Analyzer” Protocol on Icy Website
  • 01:30Opening the Protocol
  • 02:03Interacting with the Workflow on Icy
  • 02:49Merging of the Channels of an Image
  • 04:57Segmentation of the Regions of Interest
  • 07:42Fluorescent Signal Detection and Analysis
  • 09:25Run the Protocol
  • 10:35Representative Results
  • 13:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.

Tags

Substructure AnalyzerUser-friendly WorkflowRapid ExplorationAccurate AnalysisCellular BodiesFluorescence MicroscopyImage SegmentationROI AnalysisIcy SoftwareMulti-channel Image ProcessingImage PreprocessingFeature ExtractionAutomated Data Export

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