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Immunology and Infection

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JoVE Journal Immunology and Infection
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
 

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Article DOI: 10.3791/61003-v • 08:22 min • May 31st, 2020
May 31st, 2020 •
1, 2, 2, 3, 2
1Department of Microbiology, Immunology, Cancer Biology, University of Virginia, 2Cardiovascular Research Center, University of Virginia, 3Beirne B. Carter Center for Immunology Research, University of Virginia

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Here we describe a method for retroviral overexpression and adoptive transfer of murine B-1a cells to examine in vivo B-1a cell migration and localization. This protocol can be extended for diverse downstream functional assays including quantification of donor B-1a cell localization or analysis of donor cell-derived secreted factors post-adoptive transfer.

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Retroviral Overexpression CXCR4 Murine B-1a Cells Adoptive Transfer Targeted Gene Delivery B-1a Cell Migration Bone Marrow IgM Production Gene Therapeutic Potentials In Vivo Primary B-1a Cells Donor Cell Phenotype Function Post-adoptive Transfer Peritoneal Fluid Harvesting Recovery Puncturing Intestines Peritoneal Cell Population Peritoneal B-1 Cell Collection Surgical Scissors Abdomen CD45.1+apolipoprotein E Knockout Mouse Peritoneal Wall 10 Milliliter Syringe 25 Gauge Needle RPMI-1640 Medium
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