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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
 

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Article DOI: 10.3791/51189-v • 13:34 min • March 17th, 2014
March 17th, 2014 •
1, 1,2
1Department of Immunology, The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

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This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.

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Keywords: Murine Plasmacytoid Dendritic Cells Peyer's Patches Adoptive Transfer Hematopoietic Progenitors Common Dendritic Cell Progenitors Flt3 Ligand Ifnar-/- Mice Flow Cytometry
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