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Intratracheal Instillation of Stem Cells in Term Neonatal Rats
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JoVE Journal Medicina
Intratracheal Instillation of Stem Cells in Term Neonatal Rats

Intratracheal Instillation of Stem Cells in Term Neonatal Rats

DOI:
10.3791/61117-v

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04:27 min

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May 04, 2020

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, ,
1Department of Pediatrics, School of Medicine, College of Medicine,Taipei Medical University, 2Department of Pediatrics,Taipei Medical University Hospital

Capitoli

  • 00:04Introduction
  • 00:40Intratracheal Instillation
  • 02:09Pulmonary Mesenchymal Stromal Cell (MSC) Distribution Monitoring
  • 02:52Results: Representative Human MSC Characterization and Distribution
  • 03:53Conclusion

Summary

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Described is a protocol for performing intratracheal transplantation of mesenchymal stromal cells (MSCs) through intratracheal injection in term neonatal rats. This technique is a clinically viable option for delivery of stem cells and drugs into neonatal rat lungs to evaluate their efficacy.

Tags

Intratracheal InstillationStem CellsNeonatal RatsPulmonary DeliveryMesenchymal Stromal CellsLuciferaseGFPCell TransplantationImagingCell Characterization

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