Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Chemistry

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
 

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Article DOI: 10.3791/61122-v 06:19 min May 12th, 2020
May 12th, 2020

Capitoli

Riepilogo

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Tags

Keywords: TBARS Assay Oxidative Stress Biological Samples MDA Thiobarbituric Acid Sodium Hydroxide Sodium Acetate Spectrophotometric Analysis Sample Preparation Standard Curve
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter