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JoVE Journal Bioengineering
Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
 

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Article DOI: 10.3791/61154-v • 08:01 min • May 12th, 2020
May 12th, 2020 •
1, 1, 1, 2, 2, 1
1Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, 2Department of Physics, West Chester University

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Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.

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Keywords: Zero Mode Waveguides Single Molecule Microscopy Nanosphere Lithography Evaporative Deposition Polystyrene Beads Humidity Chamber Optical Borosilicate Glass Coverslips Acetone Potassium Hydroxide Ethanol Centrifugation Relative Humidity
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