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Assessment of Global Ocular Structure Following Spaceflight Using a Micro-Computed Tomography (Micro-CT) Imaging Method
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Biologia
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JoVE Journal Biologia
Assessment of Global Ocular Structure Following Spaceflight Using a Micro-Computed Tomography (Micro-CT) Imaging Method

Assessment of Global Ocular Structure Following Spaceflight Using a Micro-Computed Tomography (Micro-CT) Imaging Method

DOI:
10.3791/61227-v

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09:11 min

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October 27, 2020

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, , ,
1Center for Dental Research,Loma Linda University, 2Department of Basic Sciences, Division of Biomedical Engineering Sciences (BMES),Loma Linda University

Capitoli

  • 00:00Introduction
  • 02:10Protocol: Sample Preparation for Micro-CT Scanning
  • 03:41Micro-CT Scanning- Analysis
  • 07:36Risultati
  • 07:48Discussion
  • 08:46Conclusion

Summary

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We present a protocol using high-resolution micro-computed tomography imaging to determine whether spaceflight induced damage on ocular structures. The protocol shows the micro-CT-derived measurement of ex vivo rodent ocular structures. We demonstrate the ability to assess ocular morphological changes following spaceflight using a non-destructive tridimensional technique to evaluate ocular damage.

Tags

Micro-CTOcular StructureSpaceflightSANSRodent ModelRetinaRPEChoroidEye EnucleationDehydrationContrast Agent

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